Crown gall teratoma formation is plasmid and plant controlled.
نویسندگان
چکیده
Experiments using different species of the plant Nicotiana and strains of the bacterium Agrobacterium tumefaciens showed that teratoma formation from crown galls was dependent on the combination of bacterial Ti plasmid and host plant used.
منابع مشابه
Hormonal control of tobacco crown gall tumor morphology.
The endogenous levels of auxin and cytokinin in teratoma and unorganized tobacco (Nicotiana tabacum L. var Wisconsin #38) crown gall tumor tissues were determined. Teratoma tissues contain levels of auxin and cytokinin favorable for shoot formation, whereas unorganized tumors contain levels of auxin that suppress shoot formation. This conclusion is based upon the observation that when levels of...
متن کاملRevertant seedlings from crown gall tumors retain a portion of the bacterial Ti plasmid DNA sequences.
BT37 is a crown gall teratoma incited on tobacco by Agrobacterium tumefaciens containing pTi-T37, a nopaline-type Ti plasmid. Treatment of this cloned tumor tissue with kinetin at 1 mg/liter results in the formation of relatively normal-appearing shoots. These shoots can be induced to root and set viable seed. In contrast to BT37 tissue, the derived tissues are not phytohormone independent and ...
متن کاملShort direct repeats flank the T-DNA on a nopaline Ti plasmid.
Crown gall disease results from the insertion of a segment of the Agrobacterium Ti plasmid, called T-DNA, into host plant nuclear DNA. We have subjected to sequence analysis the border regions of pTi T37 (ends of T-DNA) and one left T-DNA/plant DNA border fragment isolated from BT37 tobacco teratoma by molecular cloning. These sequence studies, taken together with published sequence of a right ...
متن کاملEtiology of Chinaberry Gall Disease in Iran
Chinaberry (Melia azedarach L.) is a beautiful tree indigenous to the Himalayas that grows as a native plant in Iran. Symptoms similar to bacterial gall have been observed on the crown, shoot and twig of chinaberry, a recent landscape tree since 2003-2004 in Shiraz, Fars province, south of Iran. A gram negative bacterium was isolated from the galls. The isolates did not produce fluorescent pigm...
متن کاملExpression of a crown gall biological control phenotype in an avirulent strain of Agrobacterium vitis by addition of the trifolitoxin production and resistance genes
BACKGROUND Agrobacterium vitis is a causal agent of crown-gall disease. Trifolitoxin (TFX) is a peptide antibiotic active only against members of a specific group of alpha-proteobacteria that includes Agrobacterium and its close relatives. The ability of TFX production by an avirulent strain of Agrobacterium to reduce crown gall disease is examined here. RESULTS TFX was shown to be inhibitory...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 137 2 شماره
صفحات -
تاریخ انتشار 1979